Expression and Simple Purification Strategy for the Generation of Antimicrobial Active Enterocin P from Enterococcus faecium Expressed in Escherichia coli ER2566
نویسندگان
چکیده
منابع مشابه
Expression and Simple Purification Strategy for the Generation of Anti-microbial Active Enterocin P from Enterococcus faecium Expressed in Escherichia coli ER2566
Background: Enterocin-P of Enterococcus faecium P13 (EntP) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various pH, as well as its excellent thermal stability. However, recombinant production of EntP is still in laboratory because of low productivity and complex purification process. Objectives: In this study, we aimed to ...
متن کاملexpression and simple purification strategy for the generation of anti-microbial active enterocin p from enterococcus faecium expressed in escherichia coli er2566
background: enterocin-p of enterococcus faecium p13 (entp) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various ph, as well as its excellent thermal stability. however, recombinant production of entp is still in laboratory because of low productivity and complex purification process. objectives: in this study, we aimed to d...
متن کاملPURIFICATION AND CHARACTERIZATION OF THE CLONED HUMAN GM-CSF GENE EXPRESSED IN ESCHERICHIA COLI
The human granulocyte-macrophage colony stimulation factor (hGM-CSF) gene was cloned in the pET 23a( +) expression vector under the control of strong bacteriophage T7 transcription and translation signals. The hGM-CSF gene was transferred into E. coli strainBL21 (DE3)pLysS andIPTG was used for induction of GM-CSF gene. Production of the target protein was obtained as revealed by ELISA and ...
متن کاملHeterologous extracellular production of enterocin P from Enterococcus faecium P13 in the methylotrophic bacterium Methylobacterium extorquens.
Enterocin P (EntP), a strong antilisterial pediocin-like bacteriocin from Enterococcus faecium P13, was produced by Methylobacterium extorquens. For heterologous expression of EntP in the methylotrophic bacterium M. extorquens, a recombinant plasmid was constructed. The gene encoding the EntP structural gene (entP) was cloned into the plasmid vector pCM80, under control of the methanol dehydrog...
متن کاملExpression and purification of active PKB kinase from Escherichia coli.
PKB/Akt is a protein involved in control of apoptosis, proliferation and cellular metabolism, and it has been found to be activated in many cancers. Activation of PKB involves recruitment of the enzyme by its PH domain to the cell membrane, and phosphorylation at two residues, T308 and S473. To produce active PKB kinase from Escherichia coli, we constructed a derivative of PKB lacking the PH do...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2014
ISSN: 1728-3043,2322-2921
DOI: 10.15171/ijb.1154